Coding

Part:BBa_K4115001:Design

Designed by: Kaijun Wang   Group: iGEM22_ShanghaiTech_China   (2022-09-19)


superfolder GFP with LVA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It can be used as a reporter. For example, you can add it downstream an uncharacterized promoter. By measuring the fluorescence intensity, you can know the relative strength of the promoter. After adding an LVA degradation tag, its half-life decreases. So sfGFP with LVA tag can show more immediate changes in cells.


Source

sfGFP is amplified from pET21a-sfGFP with PCR. pET21a-sfGFP is a gift from Professor Hisashi Tadakuma'lab of Shanghaitech University. The LVA tag is also added through PCR. We added the LVA sequence to the 5' of the reverse primer of sfGFP.

References